Year: 2013 | Month: November | Volume 6 | Issue 4s
Characterization of Sugar Binding Lectins Isolated from Different Parts of Eudrilus Eugeniae
Abstract: <div>Lectins occur widely in animal and plant kingdom. In animals there is wide range of reports of presence of lectin in vertebrates and invertebrates, but their function is not fully understood in invertebrates. It may either have a role in the developmental stages or in the defense mechanism by providing immunity. In earthworm species like Eudrilus eugeniae presence of lectin like proteins, were reported. The aim of this study is to isolate the sugar binding lectin like proteins from Coelomocytes (CC), muscles (MC), gut/whole body (WE) & vermicompost (VC) of earthworms and characterize them. Partially purified lectins were estimated for their protein content by Biuret method and it was observed that lectins isolated from CC have more protein content of 0.178 mg/ml as compared to any other source. Their sugar binding specificity was checked by DNS method and it was observed that CC lectins</div><div>and VC lectins have more affinity for glucose (CC glu & VC glu) while MC lectin and WE lectins have more affinity for galactose (MC gal & WE gal).</div>
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
